The DNA of all eukaryotic cells is an extremely stable molecule that supports genetic information. During its replication or transcription, various events can affect its integrity. Physical or chemical agents can alter its structure. Thus, the cells can develop resistance in response to first exposure to therapeutic chemicals, or exposure to ionizing radiation. This resistance known as MDR (M = Multi D = Drug, R = Resistance), seems to be proportional to the duration of contact between the malignant cell and the repair inducer.
MDR is the expression of a characteristic mean of the cells to resist that defeat attempts at endogenous regulation (inside the body), as well as exogenous therapeutic means (outside the body). It results in excessive expression of a cell membrane protein already initiated. This protein works as a pump pushing extracellular space substances intended to act on the metabolism of anarchic cells.
Cells have many processes for conserving their genome, including homologous recombination and non-homologous recombination.
Homologous recombination is a repair pathway that covers all genetic information lost as a result of breakage. Non-homologous recombination is a repair pathway characterized by the absence of a homology search between the lost or damaged DNA strand and the newly reconstituted strand. This approximate repair is orchestrated by an enzymatic complex called DNA-protein kinase (DNA-PKcs). Non-homologous recombination is of particular interest for living beings, when the DNA of the cells has to be repaired or this molecule plays a non-dominant role (epithelial cells, red blood cells, etc.).
Our in vitro studies have made it possible to objectify the effects of Cinchona sp. combined with the standard extract of Vanilla tahitensis.
In fact, Cinchona sp. Powder, by inhibiting the operation of "pump proteins", makes it easier for repairing substances to reach the cytoplasm of the malignant cells and stay there longer. In vitro studies highlight the antimutagenic effects of the standard Vanilla tahitensis extract.
DNA-PKASE® Inhibat is a composition of plants and plant extracts intended to inhibit the non-homologous repair of DNA damage.
DNA-PKASE® Inhibat combines substances of great innocuousness in order to guide the repair of DNA to repair "ad integrum".